A micropipettor is a laboratory instrument that is used to transfer small volumes of liquid, typically in the range of 0.5–1000 μl (microliters). It is an essential tool for many scientific and medical procedures, including DNA sequencing, protein analysis, and cell culture. In this guide, we will explain how micropipettors work, how to choose the right micropipettor for your needs, and how to properly use and maintain one.

How does a micropipettor work?

Micropipettors operate on the principle of air displacement. The user selects the desired volume to be transferred, and the micropipettor draws in an appropriate amount of air. This creates a vacuum inside the pipette tip, which sucks in the liquid to be transferred. To dispense the liquid, the user simply pushes a button or lever, which releases the vacuum and allows the liquid to be expelled from the tip.

There are two main types of micropipettors: manual and electronic. Manual micropipettors require the user to manually adjust the volume by turning a dial or lever. Electronic micropipettors, on the other hand, allow the user to select the desired volume using a digital display and keypad. Electronic micropipettors are generally more precise and easier to use than manual ones, but they tend to be more expensive.

Choosing the right micropipettor

There are a few factors to consider when choosing a micropipettor. First, you need to consider the volume range that you will be working with. If you will be transferring very small volumes (e.g., less than 50 μl), you will need a micropipettor with a low volume range. For larger volumes (e.g., 1000 μl or more), you will need a micropipettor with a higher volume range.

Next, you need to consider the precision of the micropipettor. If you will be working with very small volumes or if your experiments require a high level of accuracy, you will need a micropipettor with a high precision rating. This is typically measured in terms of percent CV (coefficient of variation), with lower values indicating higher precision.

You should also consider the type of liquids you will be working with. Some micropipettors are better suited for handling viscous or volatile liquids, while others are better for handling aqueous solutions. Make sure to choose a micropipettor that is compatible with the types of liquids you will be working with.

Finally, you should consider the cost and durability of the micropipettor. Electronic micropipettors tend to be more expensive than manual ones, but they are generally more precise and easier to use. Additionally, some micropipettors are more durable and have longer lifespans than others, so be sure to consider these factors when making your decision.

Using a micropipettor

Before using a micropipettor, it is important to properly calibrate it to ensure accuracy. This can typically be done using a set of calibration standards or by following the manufacturer's instructions.

To use a micropipettor, first select the desired volume and attach a pipette tip. Then, gently touch the tip to the surface of the liquid you want to transfer, and release the vacuum by pressing the button or lever. This will draw the liquid to the tip. To dispense the liquid, place the tip in the desired location and release the vacuum again. Be sure to avoid contaminating the pipette tip by not allowing it to touch.

A laboratory's foundation is liquid handling equipment such as a micropipettor. Microlit offers a wide range of goods, including micropipettor, at competitive prices. Pipettes and many more goods may be found here.